Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2SO4 | 3.00 | g | 2.994 | 21.079 | |
| KH2PO4 | 0.20 | g | 0.2 | 1.467 | |
| NH4Cl | 0.30 | g | 0.299 | 5.597 | |
| NaCl | 21.00 | g | 20.958 | 358.626 | |
| MgCl2 x 6 H2O | 3.10 | g | 3.094 | 15.218 | |
| KCl | 0.50 | g | 0.499 | 6.694 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.15 | 1.018 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 1.50 | g | 1.497 | 14.124 | |
| Na-caproate | 1.40 | g | 1.397 | 10.114 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Na2S x 9 H2O | 0.40 | g | 0.399 | 1.662 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, caproate, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add caproate, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 7.2 - 7.4, if necessary. | |||||
Media with this solution
| 325 | SYNTROPHOSPORA MEDIUM |