Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HEPES
(SIGMA) |
11.92 | g | 11.79 | 49.477 | |
| KH2PO4 | 0.30 | g | 0.297 | 2.181 | |
| NaCl | 0.60 | g | 0.593 | 10.155 | |
| MgSO4 x 7 H2O | 0.10 | g | 0.099 | 0.401 | |
| CaCl2 x 2 H2O | 0.08 | g | 0.079 | 0.538 | |
| Trace element solution | 10.00 | ml | - | - | |
| NH4Cl | 1.00 | g | 0.989 | 18.492 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
|
2-Furoic acid
(SIGMA) |
2.24 | g | 2.216 | 19.768 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.30 | g | 0.297 | 1.689 | |
| Na2S x 9 H2O | 0.30 | g | 0.297 | 1.235 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except vitamins, furoic acid, cysteine, and sulfide, and adjust the pH of the solution to 7.0. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add furoic acid (stock solution neutralized with NaOH), cysteine, and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Before use, adjust the pH of the complete medium to 7.0 - 7.2, if necessary. | |||||
Media with this solution
| 318a | PELOBACTER (FUF) MEDIUM |