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Main sol. 315

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Internal Solution-ID: S582

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.68 g 0.68 12.713
K2HPO4 0.30 g 0.3 1.722
KH2PO4 0.18 g 0.18 1.323
(NH4)2SO4 0.15 g 0.15 1.135
MgSO4 x 7 H2O 0.12 g 0.12 0.487
CaCl2 x 2 H2O 0.06 g 0.06 0.408
FeSO4 x 7 H2O 0.02 g 0.02 0.072
Trace element solution 10.00 ml - -
Vitamin solution 10.00 ml - -
Cellobiose OR Cellulose
(cellulose: i.e. MN 300) 		5.00 g 5 14.607
Resazurin 1.00 mg 1.0e-3 0.004
NaHCO3 2.00 g 2 23.808
L-Cysteine HCl x H2O 0.25 g 0.25 1.423
Na2S x 9 H2O 0.25 g 0.25 1.041
Distilled water 1000.00 ml - -
1 Adjust pH to 7.0. Gas atmosphere: 80% N2 + 20% CO2.
2 Filter sterilize cellobiose separately.
315 BACTEROIDES CELLULOSOLVENS MEDIUM