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Main sol. 830b

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Internal Solution-ID: S5659

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.50 g 0.5 -
Proteose peptone
(Difco no. 3) 		0.50 g 0.5 -
Casamino acids 0.50 g 0.5 -
Glucose 0.50 g 0.5 2.775
Starch
(soluble) 		0.50 g 0.5 -
Na-pyruvate 0.30 g 0.3 2.726
K2HPO4 0.30 g 0.3 1.722
MgSO4 x 7 H2O 0.05 g 0.05 0.203
KNO3 0.50 g 0.5 4.945
Trace element solution SL-10 10.00 ml - -
Distilled water 1000.00 ml - -
1 Prepare liquid medium under anaerobic conditions (N2).
830b MEDIUM R2A FOR DENITRIFYING BACTERIA