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Main sol. 830a

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Internal Solution-ID: S5658

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.50 g 0.5 -
Proteose peptone
(Difco no. 3) 		0.50 g 0.5 -
Casamino acids 0.50 g 0.5 -
Glucose 0.50 g 0.5 2.775
Na-pyruvate 0.30 g 0.3 2.726
K2HPO4 0.30 g 0.3 1.722
MgSO4 x 7 H2O 0.05 g 0.05 0.203
Agar 15.00 g 15 44.599
Tween 80 1.00 ml - -
Distilled water 1000.00 ml - -
1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.
830a MEDIUM R2A MODIFIED