| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2HPO4 x 2 H2O | 4.50 | g | 4.5 | 31.699 | |
| KH2PO4 | 1.50 | g | 1.5 | 11.023 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| MnSO4 x H2O | 0.01 | g | 0.01 | 0.059 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| CaCl2 x 2 H2O | 0.01 | g | 0.01 | 0.068 | |
| Ferric ammonium citrate | 5.00 | mg | 0.005 | - | |
| Trace element solution SL-6 | 3.00 | ml | - | - | |
| Agar , for solid medium | 15.00 | g | 15 | 44.599 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 4.0. Agar if necessary 15.0 g. Distribute in 30 - 50 ml amounts in Erlenmeyer flasks and sterilize for 15 minutes at 121°C. | |||||
| 2 Incubate at 50°C without agitation under 5% O2, 10% CO2, 45% H2 (v/v). | |||||
Media with this solution
| 369 | BACILLUS TUSCIAE MEDIUM |