Equipment needed: Autoclave
Water bath
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Casein peptone
(tryptic digest) |
10 | g | 10 | - | |
| Yeast extract | 5 | g | 5 | - | |
| Glucose | 5 | g | 5 | 27.753 | |
| NaCl | 5 | g | 5 | 85.558 | |
| Agar | 15 | g | 15 | 44.599 | |
| Distilled water | 1000 | ml | - | - | |
| Defibrinated Blood | 5 | % | 50 | - | |
| 1 Sterilize medium and cool to about 45°C . Aseptically add 5% defibrinated blood. | |||||
| 2 Adjust pH to 7.2 - 7.4. | |||||
Media with this solution
| 240 | CORYNEBACTERIUM MEDIUM WITH BLOOD |