Main sol. 78d
Internal Solution-ID: S5564
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Medium 78 | 1000 | ml | - | - | |
1 To medium 78 add per liter: | |||||
Calf serum
(newborn or fetal) |
10 | ml | - | - | |
Amino acid solution | 5 | ml | - | - | |
Vitamin solution | 5 | ml | - | - | |
Hemin solution | 5 | ml | - | - | |
Peptone mixture | 5 | ml | - | - | |
Ascorbic acid solution 2%(w/v) | 5 | ml | - | - | |
KCl
(100 g/l) |
5 | ml | - | - | |
N-acetylglucosamin- and pyruvate solution | 5 | ml | - | - | |
Glutathione solution 14%(w/v) | 10 | ml | - | - | |
Sugar solution | 10 | ml | - | - | |
2 Prepare medium anaerobically under N2:CO2 (80:20). After inoculation of the tubes, pressurize them with hydrogen gas to 150 kP (1.5 bar). | |||||
3 All following solutions were filter sterilized |
78d | MEDIUM FOR TREPONEMA PARVUM |