Equipment needed: Hungate tubes
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Medium 78 | 1000 | ml | - | - | |
| 1 To medium 78 add per liter: | |||||
|
Calf serum
(newborn or fetal) |
10 | ml | - | - | |
| Amino acid solution | 5 | ml | - | - | |
| Vitamin solution | 5 | ml | - | - | |
| Hemin solution | 5 | ml | - | - | |
| Peptone mixture | 5 | ml | - | - | |
| Ascorbic acid solution 2%(w/v) | 5 | ml | - | - | |
|
KCl
(100 g/l) |
5 | ml | - | - | |
| N-acetylglucosamin- and pyruvate solution | 5 | ml | - | - | |
| Glutathione solution 14%(w/v) | 10 | ml | - | - | |
| Sugar solution | 10 | ml | - | - | |
| 2 Prepare medium anaerobically under N2:CO2 (80:20). After inoculation of the tubes, pressurize them with hydrogen gas to 150 kP (1.5 bar). | |||||
| 3 All following solutions were filter sterilized | |||||
Media with this solution
| 78d | MEDIUM FOR TREPONEMA PARVUM |