Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Modified MJ synthetic seawater C | 1000.0 | ml | - | - | |
| Ferrihydrite | 2.0 | g | 1.976 | - | |
| 1 Mix components and adjust pH to 6.5. Distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solution (filter-sterilized): | |||||
|
NaHCO3
(8%) |
12.5 | ml | - | - | |
| 2 Check final pH of the medium to be 6.5. | |||||
Media with this solution
| J1293 | MMJF MEDIUM |