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Main sol. 269
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Main sol. 269
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Internal Solution-ID: S535
Equipment needed:
Autoclave
Compound
Amount
Unit
Conc. [g/L]
Conc. [mM]
(NH
4
)
2
SO
4
2.0
g
2
15.136
KCl
0.1
g
0.1
1.341
K
2
HPO
4
0.5
g
0.5
2.871
MgSO
4
x 7 H
2
O
0.5
g
0.5
2.029
Yeast extract
0.3
g
0.3
-
D-Glucose
1.0
g
1
5.551
Distilled water
1000.0
ml
-
-
1
Autoclave glucose and yeast extract separately as a 10% and 3%, resp., stock solution. Adjust pH of complete medium to 3.0 with 1 N H
2
SO
4
.
Media with this solution
269
ACIDIPHILIUM MEDIUM
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