Equipment needed: Autoclave
Anaerobic workbench
| 1 Mineral solution: | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(BD-Difco) |
4.500 | g | 4.455 | - | |
| KH2PO4 | 1.500 | g | 1.485 | 10.913 | |
| K2HPO4 | 2.900 | g | 2.871 | 16.485 | |
| Urea | 2.900 | g | 2.871 | 47.81 | |
| L-Cysteine HCl x H2O | 3.000 | g | 2.97 | - | |
| MOPS | 20.900 | g | 20.693 | 98.882 | |
| Cellobiose | 10.000 | g | 9.901 | 28.925 | |
| Resazurin | 1.000 | mg | 9.9e-4 | 0.004 | |
| Distilled water | 1000.000 | ml | - | - | |
| MgCl2 x 6 H2O | 2.500 | g | 2.475 | 12.175 | |
| CaCl2 x 2 H2O | 0.375 | g | 0.371 | 2.526 | |
| FeSO4 x 7 H2O | 0.031 | g | 0.031 | 0.11 | |
| ZnCl2 | 10.000 | mg | 0.01 | 0.073 | |
| L-Cysteine HCl x H2O | 0.030 | g | 0.03 | - | |
| Distilled water | 10.000 | ml | - | - | |
| 2 Mix components thoroughly and adjust pH to 7.4. Distribute the medium into culture vessels under a N2-CO2 (9:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter 1 ml following sterile solution from anaerobic stock. | |||||
Media with this solution
| J965 | G-S MEDIUM |