Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Tryptone
(BD-Difco) |
1.00 | g | 0.979 | 13.779 | |
| Peptone | 1.00 | g | 0.979 | - | |
| Yeast extract | 1.00 | g | 0.979 | - | |
| K2HPO4 | 1.60 | g | 1.567 | 8.997 | |
| NaH2PO4 x 2 H2O | 1.00 | g | 0.979 | 8.164 | |
| NH4Cl | 0.50 | g | 0.49 | 9.155 | |
| MgSO4 x 7 H2O | 0.16 | g | 0.157 | 0.636 | |
| CaCl2 x 2 H2O | 0.06 | g | 0.059 | 0.4 | |
| Trace element solution SL-4 | 10.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
| Resazurin | 0.50 | mg | 4.9e-4 | 0.002 | |
| Distilled water | 930.00 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 7.0. Autoclave the medium under a N2 atmosphere. Add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized): | |||||
|
Glucose
(10%) |
50.00 | ml | - | - | |
|
NaHCO3
(*, 8%) |
1.25 | ml | - | - | |
|
L-Cysteine HCl x H2O
(3%) |
10.00 | ml | - | - | |
|
Na2S x 9 H2O
(3%) |
10.00 | ml | - | - | |
Media with this solution
| J825 | KOKO MEDIUM |