Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2SO4 | 3.0 | g | 3 | 21.121 | |
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| MgCl2 x 6 H2O | 0.2 | g | 0.2 | 0.984 | |
| KH2PO4 | 0.3 | g | 0.3 | 2.205 | |
| Na2HPO4 x 12 H2O | 2.0 | g | 2 | 14.089 | |
| Trace element solution | 10.0 | ml | - | - | |
|
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) |
1.5 | ml | 0.002 | 0.005 | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| Yeast extract | 1.0 | g | 1 | - | |
| Na-L-lactate | 4.0 | g | 4 | - | |
| Wolin's vitamin solution | 5.0 | ml | - | - | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except yeast extract, lactate, vitamins and sulfide) and sparge medium for 30 - 45 min with 100% N2 gas to make it anoxic. Distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add yeast extract, lactate, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Before use, neutralize the sodium sulfide solution by dropwise addition of 2 N H2SO4 (see medium 29). Adjust pH of complete medium to 6.8 - 7.0. | |||||
Media with this solution
| 206 | THERMODESULFOBACTERIUM MEDIUM |