Main sol. J559
Internal Solution-ID: S4381
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 13.850 | g | 13.85 | 236.995 | |
| MgSO4 x 7 H2O | 3.500 | g | 3.5 | 14.2 | |
| MgCl2 x 6 H2O | 2.750 | g | 2.75 | 13.527 | |
| KH2PO4 | 0.500 | g | 0.5 | 3.674 | |
| (NH4)2SO4 | 0.250 | g | 0.25 | 1.892 | |
| KCl | 0.330 | g | 0.33 | 4.427 | |
| CaCl2 x 2 H2O | 0.380 | g | 0.38 | 2.585 | |
| NaBr | 0.050 | g | 0.05 | 0.486 | |
| H3BO3 | 0.015 | g | 0.015 | 0.243 | |
| SrCl2 x 6 H2O | 7.500 | mg | 0.008 | 0.028 | |
| KI | 0.050 | mg | 5.0e-5 | 1.9e-4 | |
| NaHCO3 | 0.160 | g | 0.16 | 1.905 | |
|
Sulfur
(powder) |
10.000 | g | 10 | 311.818 | |
| Resazurin | 1.000 | mg | 1.0e-3 | 0.004 | |
| Na2S x 9 H2O | 0.500 | g | 0.5 | 2.082 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Mix ingredients except Na2S x 9H2O and distribute the medium into culture vessels under a stream of a N2-CO2 (4:1, v/v) gas mixture. Add Na2S x 9H2O as a 5% solution, adjust pH to 6.0 and seal with butyl rubber stoppers. Autoclave at 110C for 1 hr. Prior to cultivation, replace the gas phase with a H2-CO2 (4:1, v/v) gas mixture. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v). | |||||
Media with this solution
| J559 | 1/2 SME MEDIUM |