Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MJ(-N) synthetic seawater | 1000 | ml | - | - | |
| NaNO3 | 1 | g | 0.99 | 11.649 | |
| Na2S2O3 x 5 H2O | 1 | g | 0.99 | 3.989 | |
| NaHCO3 | 1 | g | 0.99 | 11.786 | |
| Trace vitamins | 10 | ml | - | - | |
|
Sulfur
(powder) |
3 | g | 2.97 | 92.619 | |
| 1 Mix ingredients except sulfur, adjust pH to 6.5 - 7.0 with NaOH and filter-sterilize. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) containing sulfur under a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Pressurize the inoculated bottles to 300 kPa H2-CO2 (4:1, v/v). | |||||
| 2 Comment: Adjust final pH to 7.0 (for strain JCM 12458) or 6.5 (for strain JCM 12459). | |||||
Media with this solution
| J421 | MMJHS MEDIUM |