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Main sol. J346

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Internal Solution-ID: S4066

Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(BD-Difco) 		0.50 g 0.5 -
Proteose peptone no. 3
(BD-Difco) 		0.50 g 0.5 -
Casamino acids
(BD-Difco) 		0.50 g 0.5 -
Glucose 0.50 g 0.5 2.775
Starch
(soluble) 		0.50 g 0.5 -
Sodium pyruvate 0.30 g 0.3 2.726
K2HPO4 0.30 g 0.3 1.722
MgSO4 x 7 H2O 0.05 g 0.05 0.203
Agar 15.00 g 15 44.599
Distilled water 1000.00 ml - -
1 Adjust pH to 7.2.
2 Comment: The premixed powder is available from Becton Dickinson & Co. as Difco R2A Agar.
J346 R2A AGAR