Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Starch
(soluble) |
5.0 | g | 4.926 | - | |
| KH2PO4 | 0.5 | g | 0.493 | 3.62 | |
| Trace minerals | 15.0 | ml | - | - | |
| NiCl2 x 6 H2O | 2.0 | mg | 0.002 | 0.015 | |
| NaCl | 20.0 | g | 19.704 | 337.174 | |
|
Yeast extract
(BD-Difco) |
0.5 | g | 0.493 | - | |
| Resazurin | 1.0 | mg | 9.9e-4 | 0.004 | |
| Na2S x 9 H2O | 0.5 | g | 0.493 | 2.051 | |
| Artificial sea water | 250.0 | ml | - | - | |
| Distilled water | 750.0 | ml | - | - | |
| 1 Mix components except Na2S x 9H2O, adjust pH to 6.5 and autoclave under a N2 atmosphere. Separately autoclave a 5% Na2S x 9H2O solution (pH 7.0) under a N2 atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O solution to the medium. | |||||
Media with this solution
| J237 | THERMOTOGA MEDIUM |