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Hutner's vitamin free mineral base

Internal Solution-ID: S3799

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 10.000 g 10 52.318
MgSO4 x 7 H2O 14.450 g 14.45 58.626
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 -
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
Metals ''44'' 50.000 ml - -
Distilled water 950.000 ml - -
1 After nitrilotriacetic acid is dissolved in distilled water and neutralized with ca. 7.3 g of KOH, add the remaining salts.
J149 TETRAMETHYL AMMONIUM MEDIUM