Equipment needed: Centrifuge
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
V8 canned vegetable juice
(Campbell) |
200 | ml | - | - | |
| CaCO3 | 3 | g | 3 | 29.973 | |
| Agar | 15 | g | 15 | 44.599 | |
| Distilled water | 800 | ml | - | - | |
| 1 Adjust pH to 7.2. | |||||
| 2 Filter V8 vegetable juice through 4 layers of cheesecloth or centrifuge (3,000 rpm, 10 min) to eliminate fibers. | |||||
Media with this solution
| J66 | V8 JUICE AGAR |