Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Lab-Lemco beef extract
(Oxoid) |
3.0 | g | 2.857 | - | |
|
Proteose peptone no. 3
(BD-Difco) |
10.0 | g | 9.524 | - | |
|
Trypticase peptone
(BD-BBL) |
5.0 | g | 4.762 | - | |
|
Phytone peptone
(BD-BBL) |
3.0 | g | 2.857 | - | |
|
Yeast extract
(BD-Difco) |
5.0 | g | 4.762 | - | |
|
Liver extract
(see below) |
150.0 | ml | - | - | |
| Glucose | 10.0 | g | 9.524 | 52.863 | |
|
Starch
(soluble) |
0.5 | g | 0.476 | - | |
| Solution A | 10.0 | ml | - | - | |
| Solution B | 5.0 | ml | - | - | |
| Tween 80 | 1.0 | g | 0.952 | 0.728 | |
|
Agar
(BD-Difco, Bacto ) |
15.0 | g | 14.286 | - | |
|
L-Cysteine HCl x H2O
(5%) |
10.0 | ml | - | - | |
| Horse blood | 50.0 | ml | - | - | |
| Distilled water | 825.0 | ml | - | - | |
| 1 Adjust pH to 7.2. | |||||
| 2 To prepare liver extract, put 10 g liver powder in 170 ml water, keep at 50 to 60C for 1 hr, boil for 5 min, adjust pH to 7.2 and filter. | |||||
Media with this solution
| J13 | BL AGAR (GLUCOSE BLOOD LIVER AGAR) |