Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 16.00 | g | 15.968 | 273.239 | |
| Na2SO4 | 2.50 | g | 2.495 | 17.566 | |
| CaCl2 x 2 H2O | 1.00 | g | 0.998 | 6.788 | |
| KCl | 0.45 | g | 0.449 | 6.024 | |
| NH4Cl | 0.25 | g | 0.25 | 4.664 | |
| K2HPO4 | 0.14 | g | 0.14 | 0.802 | |
| Trace elements solution (Pfennig, 1965) | 1.00 | ml | - | - | |
|
HEPES buffer
(1 M, pH 8.0) |
20.00 | ml | 4.766 | 20 | |
| MgCl2 x 6 H2O | 6.00 | g | 5.988 | 29.454 | |
| NaHCO3 | 1.00 | g | 0.998 | 11.88 | |
| Na2S2O3 x 5 H2O | 2.50 | g | 2.495 | 10.053 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Distilled water | 980.00 | ml | - | - | |
| 1 Dissolve ingredients (except HEPES buffer, magnesium chloride, bicarbonate, thiosulfate and vitamins), dispense medium in test tubes or Erlenmeyrer flasks to 20% of volume, close with screw caps and autoclave. Add HEPES buffer, magnesium chloride, bicarbonate, thiosulfate and vitamins to the autoclaved medium from sterile stock solutions sterilized by filtration. Adjust pH of complete medium to 8.0. | |||||
Media with this solution
| 1697 | THIOSOCIUS MEDIUM |