Main sol. 1669
Internal Solution-ID: S3464
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casein peptone | 5.66 | g | 5.66 | - | |
| Soy peptone | 1.00 | g | 1 | - | |
| Yeast extract | 5.00 | g | 5 | - | |
| Peptone mixture | 5.00 | g | 5 | - | |
| Glucose | 5.83 | g | 5.83 | 32.36 | |
| K2HPO4 | 0.83 | g | 0.83 | 4.765 | |
| NaCl | 1.66 | g | 1.66 | 28.405 | |
|
Tris
(hydroxymethyl aminomethane) |
3.00 | g | 3 | 24.765 | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Hemin | 0.01 | g | 0.01 | 0.015 | |
| L-Cysteine HCl x H2O | 0.40 | g | 0.4 | 2.277 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Boil medium under CO2 until the resazurin indicator changes from pink to colourless. Cool down under CO2 and adjust pH to 7.6. Switch to N2 to prevent further lowering of the pH. Fill up into Hungate tubes under N2 and autoclave. | |||||
Media with this solution
| 1669 | SCHAEDLER BROTH (Roth; 5772) |