Equipment needed: Gassing station
Hungate tubes
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casitone | 10.00 | g | 10 | - | |
| Yeast extract | 2.50 | g | 2.5 | - | |
| Glucose | 5.00 | g | 5 | 27.753 | |
| MgSO4 x 7 H2O | 45.00 | mg | 0.045 | 0.183 | |
| CaCl2 x 2 H2O | 90.00 | mg | 0.09 | 0.612 | |
| K2HPO4 | 0.45 | g | 0.45 | 2.584 | |
| KH2PO4 | 0.45 | g | 0.45 | 3.307 | |
| NaCl | 0.90 | g | 0.9 | 15.4 | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except NaHCO3, hemin, cysteine in water and boil for 10 min, then add fatty acids: | |||||
| Volatile fatty acids | 2.70 | ml | - | - | |
| 2 Cool down to room temperature while gassing with 100% CO2. Add the NaHCO3, hemin, cysteine. | |||||
| NaHCO3 | 4.00 | g | 4 | 47.615 | |
| L-Cysteine HCl | 1.00 | g | 1 | 6.344 | |
| Hemin | 10.00 | mg | 0.01 | 0.015 | |
| 3 Adjust pH to 6.7-6.8. | |||||
Media with this solution
| 1611 | YCFA MEDIUM (modified) |