| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Peptone
(Bacto) |
0.25 | g | 0.25 | - | |
|
Yeast extract
(Bacto) |
0.25 | g | 0.25 | - | |
| HEPES | 2.38 | g | 2.38 | 9.987 | |
| Mineral salts solution | 20.00 | ml | - | - | |
| Trace element solution | 1.00 | ml | - | - | |
| Artifical sea water | 250.00 | ml | - | - | |
|
Agar
(Bacto) |
15.00 | g | 15 | 44.599 | |
| Double distilled water | 730.00 | ml | - | - | |
| 1 Sterilize 20 min./121°C. After cooling to 60°C add: | |||||
|
N-Acetylglucosamine
(10% stock solution, 10 % stock solution) |
8.00 | ml | 0.8 | 3.616 | |
|
Glucose
(2.5%, sterile-filtered) |
10.00 | ml | 0.25 | 1.388 | |
|
Vitamin solution (double conc.)
(medium 621) |
5.00 | ml | - | - | |
| 2 Adjust pH to 7.5 (the medium is only weakly buffered; one needs approx. 10 drops/l medium of 6 N KOH). | |||||
Media with this solution
| 1560 | MARINES PLANCTOMYCES MEDIUM |