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Main sol. 1511

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Internal Solution-ID: S3121

Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.33 g 0.33 2.425
NH4Cl 0.33 g 0.33 6.169
MgCl2 1.20 mg 0.001 0.013
CaCl2 1.20 mg 0.001 0.011
KCl 0.33 g 0.33 4.427
Trace Element Solution 1.00 ml - -
Wolin's vitamin solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Prepare the liquid medium. Dispense the medium into culture vessels. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:
KNO3 1.00 g 1 9.891
Glucose 2.00 g 2 11.101
Wolin's vitamin solution 1.00 ml - -
NaHCO3
(8% solution) 		12.50 ml 1 11.904
2 The 8% NaHCO3 solution may be either filter sterilized or autoclaved. When the solution is autoclaved use tightly closed vessels that are filled to a maximum of 50% of the total volume.
3 The final pH of the medium should be adjusted to pH 7.6-8.0.
1511 PLANTOMYCETACEAE MEDIUM SBP2