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Main sol. 1494

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Internal Solution-ID: S3085

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 100 ml - -
Solution A 10 ml - -
Solution B 10 ml - -
Solution C 10 ml - -
Solution D 10 ml - -
Solution E 10 ml - -
Solution F 10 ml - -
Solution G 10 ml - -
Solution H 200 µl - -
Solution I 8 µl - -
Solution J 2 µl - -
Solution K 40 µl - -
Solution L 2 ml - -
Trace elements I
(pre-dilute 1:1000; use) 		2 ml - -
Trace elements II
(pre-dilute 1:1000; use) 		2 ml - -
Trace elements III
(pre-dilute 1:1000; use) 		200 µl - -
Distilled water
(add up to 200 ml) 		200 ml - -
Supplement 1 200 µl - -
Supplement 2 2 ml - -
Supplement 3 2 ml - -
Supplement 4 200 µl - -
Supplement 5 2 ml - -
1 Add 10 ml rabbit serum, flush the medium with N2CO2, adjust the pH to 6.9 and filter-sterilize the completed medium into tubes.
Rabbit serum 10 ml - -
2 This recipe describes the preparation of 200 ml medium, which can be used for about 4 weeks when kept at 4°C.
1494 . OMIZ-PAT Medium (modified)