Equipment needed: Filter
Centrifuge
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(fresh) |
42 | g | 300 | - | |
| Distilled water | 140 | ml | - | - | |
| 1 Homogenize 42 g fresh baker yeast in 140 ml distilled water, incubate for 24h at 56°C and centrifuge to harvest the supernatant. Adjust pH to 7 with NaOH, make anoxic, filter-sterilize and store at -80°C | |||||
Media with this solution
| 1494 | . OMIZ-PAT Medium (modified) |