Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaH2PO4 | 1.380 | g | 1.38 | 11.502 | |
| NaCl | 3.000 | g | 3 | 51.335 | |
| NH4Cl | 0.107 | g | 0.107 | 2 | |
| CaCl2 x 2 H2O | 0.015 | g | 0.015 | 0.102 | |
| MgSO4 x 7 H2O | 0.123 | g | 0.123 | 0.499 | |
| KCl | 0.150 | g | 0.15 | 2.012 | |
| Na2SO4 | 0.300 | g | 0.3 | 2.112 | |
| Mineral solution | 5.000 | ml | - | - | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Adjust pH to 7.2. | |||||
| 2 Prepare the medium under a nitrogen atmosphere. Dispense 5 ml into 25 ml tubes fitted with rubber stoppers. | |||||
| 3 Autoclave at 121 °C for 15 min. | |||||
| 4 after autoklaving, add the following from anaerobic, sterile (autoclaved) stock solutions (e.g., 100x) | |||||
| Sodium acetate trihydrate | 2.000 | mM | 0.272 | 2 | |
| Sodium thiosulfate pentahydrate | 2.000 | mM | 0.496 | 2 | |
| Sodium bicarbonate | 1.000 | mM | - | 1 | |
| 5 Before inoculation 2% sterile O2 or 10% sterile air is added to the headspace. | |||||
Media with this solution
| 1486 | GBS salts modified for Thermocrinis jamiesonii |