Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 0.3 | g | 0.299 | 1.719 | |
| KH2PO4 | 0.3 | g | 0.299 | 2.2 | |
| NH4Cl | 1.0 | g | 0.998 | 18.658 | |
| MgCl2 x 6 H2O | 1.5 | g | 1.497 | 7.363 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.679 | |
| NaCl | 20.0 | g | 19.96 | 341.548 | |
| KCl | 0.1 | g | 0.1 | 1.339 | |
| Trace element solution | 1.0 | ml | - | - | |
| Yeast extract | 1.0 | g | 0.998 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| NaHCO3 | 0.5 | g | 0.499 | 5.94 | |
| D-Glucose | 3.6 | g | 3.593 | 19.942 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except bicarbonate, glucose and vitamins, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose and vitamins (sterilized by filtration) from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.0 - 7.2, if necessary. | |||||
Media with this solution
| 1477 | CALORAMATOR QUIMBAYENSIS MEDIUM |