| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 0.50 | g | 0.5 | - | |
| Yeast extract | 0.25 | g | 0.25 | - | |
| Glucose | 0.10 | g | 0.1 | 0.555 | |
|
MES
(10 mM final concentration) |
1.95 | g | 1.95 | 9.988 | |
| SSE (double concentrated) | 500.00 | ml | - | - | |
| Distilled water | 500.00 | ml | - | - | |
| 1 Add to 1000 ml of medium after autoclaving: | |||||
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Vitamin solution | 1.00 | ml | - | - | |
| 2 adjust pH to 5.5 | |||||
| 3 For solid medium use purified agar (Oxoid LP0028) which has to be autoclaved separately. | |||||
Media with this solution
| 1426 | SSE/HD 1:10 MEDIUM |