Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 20.00 | g | 20 | 342.231 | |
| K2HPO4 | 0.09 | g | 0.09 | 0.517 | |
| KH2PO4 | 0.07 | g | 0.07 | 0.514 | |
| CaCl2 x 2 H2O | 0.80 | g | 0.8 | 5.442 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| NaNO3 | 1.00 | g | 1 | 11.765 | |
| MgSO4 x 7 H2O | 4.00 | g | 4 | 16.229 | |
| MgCl2 x 6 H2O | 3.00 | g | 3 | 14.756 | |
| KCl | 0.33 | g | 0.33 | 4.427 | |
| FeSO4 x 7 H2O | 0.01 | g | 0.01 | 0.036 | |
| Na2S2O3 x 5 H2O | 2.00 | g | 2 | 8.058 | |
| Fe(III) citrate | 0.01 | g | 0.01 | 0.041 | |
| Yeast extract | 2.00 | g | 2 | - | |
| Trace mineral solution | 5.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the medium under N2/CO2 (80%N2, 20%CO2) and fill in , the pH of the medium is adjusted with NaOH to 6.8. After autoclaving, the separately autoclaved, concentrated solutions including each of follows are added to the medium. Then a mix gas (80%H2, 20%CO2) is purged for 5 min. | |||||
| 2 Finally, the mix gas (77%H2, 20%CO2, 3%O2) is compressed into gas phase (>80% volume of the tube or bottle) at 2 atm. | |||||
| Wolin's vitamin solution | 1.00 | ml | - | - | |
| NaHCO3 | 0.10 | % | 1 | 11.904 | |
Media with this solution
| 1391 | MMJHSY MEDIUM (modified) |