Equipment needed: Gassing station
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Trypticase peptone | 10.00 | g | 10 | - | |
| Yeast extract | 5.00 | g | 5 | - | |
| Glucose | 0.25 | g | 0.25 | 1.388 | |
| Cellobiose | 0.25 | g | 0.25 | 0.73 | |
| Maltose | 0.25 | g | 0.25 | 0.73 | |
|
Starch
(soluble) |
0.25 | g | 0.25 | - | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
|
Sodium resazurin
(0.1% w/v) |
1.00 | ml | 1.0e-3 | - | |
| Solution I | 75.00 | ml | - | - | |
| Solution II | 75.00 | ml | - | - | |
|
Na2CO3
(8% w/V) |
2.50 | ml | 0.2 | 1.887 | |
| Vitamin B12 | 10.00 | µg | 1.0e-5 | 7.4e-6 | |
| Distilled water | 846.50 | ml | - | - | |
| 1 The cysteine, Na2CO3, and vitamin B12 are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.3 Distribute under N2 and autoclave. | |||||
Media with this solution
| 1308 | PY4S |