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Solution A

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Internal Solution-ID: S2528

Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 27.50 g 30.522 522.273
MgCl2 x 6 H2O 5.38 g 5.971 29.371
MgSO4 x 7 H2O 6.78 g 7.525 30.53
KCl 0.72 g 0.799 10.719
CaCl2 x 2 H2O 1.40 g 1.554 10.569
NH4Cl 1.00 g 1.11 20.749
K2HPO4 0.05 g 0.055 0.319
Wolfe's mineral elixir 1.00 ml - -
NaHCO3
(for top layer) 		0.50 g 0.555 6.606
Agarose
(for top layer, low melt) 		1.50 g 1.665 2.64
Distilled water 900.00 ml - -
1267a MARIPROFUNDUS (ES) MEDIUM