Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.5 | g | 0.5 | 9.348 | |
| KH2PO4 | 0.5 | g | 0.5 | 3.674 | |
| NaCl | 25.0 | g | 25 | 427.789 | |
| Na2SO4 | 0.5 | g | 0.5 | 3.52 | |
| MgSO4 x 7 H2O | 0.2 | g | 0.2 | 0.811 | |
| KCl | 0.5 | g | 0.5 | 6.707 | |
| Yeast extract | 1.0 | g | 1 | - | |
| Peptone | 1.0 | g | 1 | - | |
| Sodium DL-lactate | 1.0 | g | 1 | 8.924 | |
| Vitamin B12 | 15.0 | µg | 1.5e-5 | 1.1e-5 | |
| Na2CO3 | 5.0 | g | 5 | 47.175 | |
| NaHCO3 | 5.0 | g | 5 | 59.519 | |
| Trace element solution SL-10 B | 1.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| Agar , for solid medium | 15.0 | g | 15 | 44.599 | |
| 1 Autoclave the Na2CO3, NaHCO3 and the lactate separately from the medium and combine after cooling. Vitamin B12 is added from a sterile stock solution. The final pH of the medium should be 9.5. Add 15.0 g/l agar for solid media. | |||||
Media with this solution
| 1212 | MARINOSPIRILLUM CELERE |