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Main sol. 1122a

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Internal Solution-ID: S2256

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 2.000 g 2 15.136
KH2PO4 2.000 g 2 14.697
NaCl 0.500 g 0.5 8.556
MgSO4 x 7 H2O 0.025 g 0.025 0.101
FeSO4 x 7 H2O 2.000 mg 0.002 0.007
Agar
(Difco) 		20.000 g 20 59.465
Distilled water 1000.000 ml - -
Methanol 0.500 %(v/v) - -
Vitamin B12
(10µg /ml, filter sterilized) 		0.100 ml - -
1 Adjust to pH 7.2.
2 After autoclaving add methanol (sterilized separately) to a concentration of 0.5 % (v/v) and 0.1 ml vitamin solution B12 (10µg /ml, filter sterilized).
1122a METHYLOVERSATILIS THERMOTOLERANS MEDIUM