Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.5 | g | 0.499 | 9.329 | |
| KCl | 0.2 | g | 0.2 | 2.677 | |
| MgCl2 x 6 H2O | 0.1 | g | 0.1 | 0.491 | |
| KH2PO4 | 0.2 | g | 0.2 | 1.467 | |
| NaCl | 1.0 | g | 0.998 | 17.077 | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
| Selenite-tungstate solution | 1.0 | ml | - | - | |
| Na2CO3 | 3.0 | g | 2.994 | 28.249 | |
| NaHCO3 | 10.0 | g | 9.98 | 118.8 | |
|
Sulfur
(powdered) |
10.0 | g | 9.98 | 311.195 | |
| Yeast extract | 0.1 | g | 0.1 | - | |
| Na-acetate | 2.5 | g | 2.495 | 30.414 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (ecxept carbonate, hydrogencarbonate, sulfur, yeast extract and acetate), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add solid carbonate and bicarbonate, adjust pH to 9.0 - 9.2, and dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur. Sterilize medium by autoclaving at 110°C for 20 min. Add yeast extract and acetate from sterile anoxic stock solutions prepared under 100% N2 gas. | |||||
Media with this solution
| 1059 | GEOALKALIBACTER MEDIUM |