Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Tryptone
(BD BBL) |
4.0 | g | 3.992 | - | |
|
Beef extract
(BD BBL) |
2.0 | g | 1.996 | - | |
|
Yeast extract
(OXOID) |
1.0 | g | 0.998 | - | |
| NaCl | 4.0 | g | 3.992 | 68.31 | |
| K2HPO4 | 1.5 | g | 1.497 | 8.595 | |
| FeSO4 x 7 H2O | 0.1 | g | 0.1 | 0.359 | |
| MgCl2 x 6 H2O | 0.2 | g | 0.2 | 0.982 | |
| Trace element solution | 1.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.499 | 2.841 | |
| D-Glucose | 10.0 | g | 9.98 | 55.395 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except cysteine, glucose and vitamins), sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add cysteine and adjust pH to 6.0, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add glucose and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Adjust pH of complete medium to 6.0, if necessary. | |||||
Media with this solution
| 1057 | ETHANOLIGENES MEDIUM |