Equipment needed: Autoclave
Hungate tubes
Gassing station
Centrifuge
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.50 | g | 0.5 | 3.674 | |
| MgSO4 x 7 H2O | 0.40 | g | 0.4 | 1.623 | |
| NaCl | 0.40 | g | 0.4 | 6.845 | |
| NH4Cl | 0.40 | g | 0.4 | 7.478 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.34 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| FeSO4 x 7 H2O solution (0.1% w/v) | 2.00 | ml | - | - | |
|
Yeast extract
(OXOID) |
1.00 | g | 1 | - | |
| Na-acetate | 1.00 | g | 1 | 12.19 | |
| Na-formate | 2.00 | g | 2 | 29.409 | |
| Sludge fluid | 50.00 | ml | - | - | |
| Fatty acid mixture | 20.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| NaHCO3 | 0.50 | g | 0.5 | 5.952 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
| Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
| Distilled water | 930.00 | ml | - | - | |
| 1 Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate, adjust pH to 6.8 and dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of complete medium and adjust to 6.8 - 7.0, if necessary. | |||||
Media with this solution
| 119c | GUDONGELLA MEDIUM |