Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea Salt
(SIGMA, must not show signs of caking) |
35.0 | g | 34.93 | - | |
| Wolfe's mineral elixir | 1.0 | ml | - | - | |
| Yeast extract | 1.0 | g | 0.998 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| NaHCO3 | 2.0 | g | 1.996 | 23.76 | |
| Na-acetate | 1.6 | g | 1.597 | 19.465 | |
| Na2-fumarate | 3.2 | g | 3.194 | 19.956 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| Na2S x 9 H2O | 0.3 | g | 0.299 | 1.247 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except bicarbonate, acetate, fumarate, vitamins, and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add acetate, fumarate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of vitamins and fumarate should be sterilized by filtration. Adjust pH of the complete medium to 7.0-7.2, if necessary. | |||||
Media with this solution
| 1041 | DESULFUROMUSA FERRIREDUCENS MEDIUM |