Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea Salt
(SIGMA, must not show signs of caking) |
35.0 | g | 34.93 | - | |
| Wolfe's mineral elixir | 1.0 | ml | - | - | |
| Yeast extract | 1.0 | g | 0.998 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 1.0 | g | 0.998 | 9.416 | |
| Na-DL-lactate | 2.5 | g | 2.495 | 22.265 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| Na2S x 9 H2O | 0.3 | g | 0.299 | 1.247 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, lactate, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add lactate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. The vitamin solution should be sterilized by filtration. Adjust pH of the complete medium to 7.0-7.2, if necessary. | |||||
Media with this solution
| 1040 | MARIDESULFOVIBRIO MEDIUM |