Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nitrilotriacetic acid
(C6H9NO6) |
0.10 | g | 0.1 | 0.523 | |
| CaSO4 x 2 H2O | 0.06 | g | 0.06 | 0.348 | |
| MgSO4 x 7 H2O | 0.10 | g | 0.1 | 0.406 | |
| NaCl | 8.00 | mg | 0.008 | 0.137 | |
| KNO3 | 0.10 | g | 0.1 | 0.989 | |
| NaNO3 | 0.69 | g | 0.69 | 8.118 | |
| Na2HPO4 | 0.10 | g | 0.1 | 0.704 | |
| Traceelements Solution | 10.00 | ml | - | - | |
|
FeCl3 x 6 H2O
(0.17 mM) |
10.00 | ml | 4.6e-4 | 0.002 | |
|
Tryptone
(Bacto Trypton 211705) |
1.00 | g | 1 | 14.069 | |
|
Yeast extract
(Oxoid LP0021, BD 212750) |
1.00 | g | 1 | - | |
| Agar | 15.00 | g | 15 | 44.599 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve the nitrilotriacetic acid (C6H9NO6), adjust the pH to 8.0 with 1 M NaOH. | |||||
| 2 Dissolve the remaining ingredients in the nitrilotriacetic acid solution and adjust to pH 8.2 with 1 M NaOH. | |||||
| 3 Autoclave at 121° C for 15 minutes. | |||||
Media with this solution
| 1033 | THERMUS MEDIUM |