| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 12.80 | g | 12.8 | 66.967 | |
| CoCl2 x 6 H2O | 0.17 | g | 0.17 | 1.309 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| MnCl2 x 4 H2O | 0.10 | g | 0.1 | 0.505 | |
| NaCl | 0.10 | g | 0.1 | 1.711 | |
| Na2MoO4 x 2 H2O | 0.10 | g | 0.1 | 0.413 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve the nitrilotriacetic acid and adjust the pH to 6.5 with KOH, then add the minerals. The final pH is 7.0, adjusted with KOH. | |||||
| 1019 | ROUF'S MEDIUM |