Equipment needed: Autoclave
Filter
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea Salt
(SIGMA) |
30.00 | g | 29.97 | - | |
| NH4Cl | 1.00 | g | 0.999 | 18.676 | |
| K2HPO4 | 0.30 | g | 0.3 | 1.721 | |
| KH2PO4 | 0.30 | g | 0.3 | 2.202 | |
| Peptone | 0.50 | g | 0.5 | - | |
| Yeast extract | 0.50 | g | 0.5 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| NaHCO3 | 2.00 | g | 1.998 | 23.784 | |
| D-Glucose | 4.00 | g | 3.996 | 22.18 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.25 | g | 0.25 | 1.422 | |
| Na2S x 9 H2O | 0.25 | g | 0.25 | 1.04 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except bicarbonate, glucose, vitamins, cysteine and sulfide), sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins, glucose, sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins should be sterilized by filtration. The pH of the complete medium should be 7.0. | |||||
Media with this solution
| 986 | PARAMALEDIVIBACTER MEDIUM |