Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 0.30 | g | 0.3 | 1.722 | |
| KH2PO4 | 0.30 | g | 0.3 | 2.205 | |
| MgCl2 x 6 H2O | 0.20 | g | 0.2 | 0.984 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| KCl | 0.10 | g | 0.1 | 1.341 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| Succinic acid | 2.36 | g | 2.36 | 19.985 | |
| Yeast extract | 1.00 | g | 1 | - | |
| Wolin's vitamin solution | 5.00 | ml | - | - | |
| Modified Wolin's mineral solution II | 10.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the medium without the vitamins. | |||||
| 2 Adjust pH to 7.0 with 10M KOH. | |||||
| 3 Autoclave at 121 °C for 20 min. | |||||
| 4 After cooling add vitamins from a filter sterilised stock solution. | |||||
Media with this solution
| 979 | PETROBACTER SUCCINIMANDENS MEDIUM |