Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 0.10 | g | 0.1 | 0.755 | |
| MgSO4 | 25.00 | mg | 0.025 | 0.207 | |
| NaCl | 90.00 | g | 89.82 | 1536.97 | |
| K2HPO4 | 0.15 | g | 0.15 | 0.859 | |
| KH2PO4 | 0.08 | g | 0.08 | 0.587 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| NaNO3 | 1.25 | g | 1.248 | 14.677 | |
| Na-DL-lactate | 1.70 | g | 1.697 | 15.14 | |
| Yeast extract | 0.20 | g | 0.2 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 10.60 | g | 10.579 | 99.812 | |
| NaHCO3 | 4.20 | g | 4.192 | 49.896 | |
| L-Cysteine HCl x H2O | 0.25 | g | 0.25 | 1.421 | |
| Na2S x 9 H2O | 0.25 | g | 0.25 | 1.039 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, hydrogencarbonate and reducing agents), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add solid carbonate and bicarbonate, adjust pH to 9.8, dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before inoculation, add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 9.8, if necessary. | |||||
Media with this solution
| 968 | ANAEROBACILLUS ARSENICISELENATIS MEDIUM |