MediaDive DSMZ Digital Diversity
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder SPARQL endpoint Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 963

Download this solution as

CSV JSON PDF

Internal Solution-ID: S1987

Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.78 g 0.772 4.43
KH2PO4 0.75 g 0.742 5.451
Na3-EDTA 0.04 g 0.04 0.11
FeSO4 x 7 H2O 0.01 g 0.01 0.036
MgSO4 x 7 H2O 0.25 g 0.247 1.003
CaCl2 x 2 H2O 0.03 g 0.03 0.202
NaCl 0.20 g 0.198 3.385
NH4Cl 0.50 g 0.495 9.246
D-Glucose 0.50 g 0.495 2.745
Yeast extract
(OXOID) 		0.50 g 0.495 -
Trace element solution 10.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
NaHCO3 5.00 g 4.946 58.871
Na2S2O3 x 5 H2O 1.24 g 1.227 4.942
Wolin's vitamin solution (10x) 1.00 ml - -
L-Cysteine HCl x H2O 0.25 g 0.247 1.408
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except bicarbonate, thiosulfate, vitamins and cysteine), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add solid bicarbonate and adjust pH to 6.8 - 7.0. Dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add thiosulfate, vitamins and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of thiosulfate and vitamins should be sterilized by filtration. Adjust pH of complete medium to 6.5, if necessary.
963 THERMOANAEROMONAS MEDIUM