Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea Salt
(SIGMA, must not show signs of caking) |
30.0 | g | 30 | - | |
|
PIPES
(SIGMA) |
6.0 | g | 6 | 19.843 | |
| Yeast extract | 1.0 | g | 1 | - | |
|
Tryptone
(BD Bacto) |
1.0 | g | 1 | 14.069 | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| D-Glucose | 2.5 | g | 2.5 | 13.877 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except glucose, sulfide and cysteine, adjust pH to 7.0 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials (to 30% of volume) and autoclave. Add glucose, sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. | |||||
Media with this solution
| 904 | MARINITOGA MEDIUM |