Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.20 | g | 0.2 | 1.467 | |
| MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.491 | |
| KCl | 0.20 | g | 0.2 | 2.677 | |
| NH4Cl | 1.00 | g | 0.998 | 18.658 | |
| NaCl | 15.00 | g | 14.97 | 256.161 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 2.76 | g | 2.754 | 25.989 | |
| NaHCO3 | 10.00 | g | 9.98 | 118.8 | |
| Yeast extract | 0.50 | g | 0.499 | - | |
| D-Glucose | 5.00 | g | 4.99 | 27.698 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Na2S x 9 H2O | 0.50 | g | 0.499 | 2.078 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve all ingredients except carbonates, yeast extract, glucose, vitamins and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add carbonate and hydrogencarbonate, adjust pH to 8.0 - 8.5, then dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add yeast extract, glucose, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. Adjust pH of complete medium to 8.5 - 9.0. | |||||
Media with this solution
| 898a | ANAEROBACILLUS MEDIUM |