Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase peptone
(BD BBL) |
5.0 | g | 5 | - | |
|
Meat peptone
(pepsin-digested) |
5.0 | g | 5 | - | |
| Yeast extract | 10.0 | g | 10 | - | |
| Salt solution | 40.0 | ml | - | - | |
| NaCl | 60.0 | g | 60 | 1026.69 | |
| MgSO4 x 7 H2O | 5.0 | g | 5 | 20.286 | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| Betaine x H2O | 6.0 | g | 6 | 51.218 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Distilled water | 960.0 | ml | - | - | |
| 1 Dissolve ingredients (except betaine and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Adjust the pH to 7.2. Thereafter, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add betaine and cysteine from sterile, anoxic stock solutions prepared under 100% N2. Adjust the pH of the complete medium to 7.3 - 7.5. | |||||
Media with this solution
| 104d | MALEDIVIBACTER MEDIUM |