Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 29.00 | g | 28.856 | 493.767 | |
| (NH4)2SO4 | 1.00 | g | 0.995 | 7.53 | |
| MgSO4 x 7 H2O | 1.50 | g | 1.493 | 6.056 | |
| CaCl2 x 2 H2O | 0.42 | g | 0.418 | 2.843 | |
| KCl | 0.70 | g | 0.697 | 9.343 | |
| Trace element solution SL-11 | 1.00 | ml | - | - | |
|
Bromothymol blue
(0.1% w/v) |
4.00 | ml | 0.004 | 0.006 | |
| K2HPO4 | 0.50 | g | 0.498 | 2.856 | |
| Na2S2O3 x 5 H2O | 5.00 | g | 4.975 | 20.046 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except hydrogenphosphate and thiosulfate, adjust pH to 6.5, then dispense medium into culture vessels and autoclave. Add hydrogenphosphate and thiosulfate from sterile stock solutions. Adjust pH of complete medium to 6.5 - 7.0. | |||||
Media with this solution
| 864 | HALOTHIOBACILLUS MEDIUM |